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2012 1

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Botrytis cinerea 1

Trichoderma harzianum 1

Trichoderma reesei 1

Box-Behnken design 1

anthraquinone 1

cellulase 1

genetic algorithm 1

harzianolide 1

harzianopyridone 1

molecular docking 1

pineapple waste 1

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Optimization of cellulase production using

Saravanan P, Muthuvelayudham R, Rajesh Kannan R, Viruthagiri T

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 4,   Pages 443-452 doi: 10.1007/s11705-012-1225-1

Abstract: The potential of for cellulase production using pineapple waste as substrate has been investigated. A maximum cellulase activity of 9.23 U/mL is obtained under the optimum experimental conditions: pH (5.5), temperature (37.5°C), initial substrate concentration (3%), inoculum concentration (6.6 × 10 ?CFU/mL), and culture time (6 days). Box-Behnken design (BBD) statistical tool and genetic algorithm (GA) are used to optimize the process parameters. The BBD study of linear and quadratic interactive effects of experimental variables on the desired response of cellulase activity showed that the second order polynomial is significant ( = 0.9414). The experimental cellulase activity under the optimal conditions identified by the BBD is 9.23 U/mL and that by GA is 6.98 U/mL. This result indicates that the BBD model gives better result than GA in the present case.

Keywords： cellulase     pineapple waste     Trichoderma reesei     Box-Behnken design     genetic algorithm    

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Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 2,   Pages 271-279 doi: 10.15302/J-FASE-2018214

Abstract: This study reports a strain of CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, , and with a pivotal role in tomato ( ) plant growth enhancement. A total of 254 isolates were screened by antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of . The antagonistic assay and greenhouse experiments indicated that CCTCC-SBW0162 gave the highest inhibition rate (90.6%) and disease reduction (80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against , molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of . The molecular docking results indicated that the combination of harzianopyridone, harzianolide and anthraquinone C derived from CCTCC-SBW0162 could synergistically improve antifungal activity against through the inhibition/modification of pathogenicity and virulence related proteins. However, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect derived metabolites against the proteins of and their interactions.

Keywords： anthraquinone     Botrytis cinerea     harzianolide     harzianopyridone     molecular docking     Trichoderma    

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Title Author Date Type Operation

Optimization of cellulase production using

Saravanan P, Muthuvelayudham R, Rajesh Kannan R, Viruthagiri T

Journal Article

Triggering the biocontrol of

Kandasamy SARAVANAKUMAR, Zhixiang LU, Hai XIA, Meng WANG, Jianan SUN, Shaoqing WANG, Qiang-qiang WANG, Yaqian LI, Jie CHEN

Journal Article